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Biological Molecules - You Are What You Eat: Crash Course Biology #3

FAQ Policy. About this book New techniques and updated protocols for the detection and analysis of biomolecules - nucleic acids, proteins and glycoproteins - are presented in the second edition of this successful laboratory manual.

Non-radioactive Protein Phosphorylation Analysis

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Varpu Marjomaki. Koshi Imami Tomoharu Yasuda. Koshi Imami imami.

Tomoharu Yasuda. Original research article A brief version of this protocol appeared in:. Similar Protocols. Reproducibility Feedback. Share your feedback.

New complexing agent for one-step Al18F-labeling of heat-sensitive biomolecules: RESCA1

Abstract Protein synthesis is one of the most fundamental biological processes to maintain cellular proteostasis. Keywords : Protein synthesis, Translation, AHA, Click chemistry, Flow cytometry, Cell cycle, Mitosis, Non-radioactive amino acid Background Traditionally measurement of protein synthesis is performed by pulse labeling of translation products using radiolabeled amino acids such as [ 35 S]methionine and [ 35 S]cysteine but the use of radioactive materials is the major disadvantage.

Materials and Reagents Pipette tips Eppendorf, epT. Mouse B cells in suspension were used in this study but any cell types can be used.